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Image Search Results
Journal: bioRxiv
Article Title: Impaired oxidative phosphorylation drives primary tumor escape and metastasis
doi: 10.1101/2025.01.08.631936
Figure Lengend Snippet: (A) Western blot analysis of the yeast complex I protein NDI1 stable expression in wild-type RENCA cells. Western blot analysis of ECAD protein expression in 40 µg of protein lysed from EV and NDI1 expressing RENCA cells. (B) Abdominal MRI demonstrating a peri-renal lymph node metastasis in a 70-year-old male HLRCC patient. Mitochondria imaged using Transmission Electron Microscopy (TEM) in (C) normal adjacent kidney and (D) tumor from the HLRCC patient in (B). Scale bars are 0.5 µm. (E) Tumor cell culture from patient in (B), designated as the continuous tumor cell line UOK365. (F) Seahorse flux analyses of FH -/- UOK262 and UOK365 tumor cells and FH +/+ control cells. (G) GSEA Hallmark analysis of bulk-RNA sequencing of 17 primary HLRCC tumors, 12 HLRCC metastatic tumors, 6 HLRCC normal adjacent kidneys, and 12 normal kidneys from donors. While (H) oxidative phosphorylation was enriched in normal kidney compared with HLRCC tumor, (I) glycolysis and (J) EMT were enriched in tumors compared with normal kidneys.
Article Snippet: NDUFA4L2 and
Techniques: Western Blot, Expressing, Transmission Assay, Electron Microscopy, Cell Culture, Control, RNA Sequencing Assay